ANTIHYPERTENSIVE ACTIVITY OF SEPAT LEAF ETHANOL EXTRACT (MITRAGYNA SPECIOSA KORTH.) ON WHITE RAT

Abstract

Empirically, sepat leaf is used toreduce blood pressure. Therefore research will be scientifically tested by in vivo method.The purpose of this research is to prove that sepat leaf ethanol extract has an effect to reduce blood pressure. In this research using 2 methods namely diuretic method and blood pressure measurement using CODA®. In the diuretic method using 25 rats grouped into 5 groups, each group was given 0.9% NaCl and each of them were given Na CMC 0.5%, furosemide 3.6 mg/KgBW, sepat leaf extract doses of 25, 50 and 100 mg/KgBW. Parameters which were seen was urine volume for 8 hours and 24 hours. In the CODA® method, 30 rats were grouped into 6 groups which was given concurrent between therapy and 25% fructose induction in drinks for 21 days. The therapy was given captopril 2.5 mg/KgBW, sepat leaf ethanol extract with doses of 25, 50 and 100 mg/KgBW.The parameters those measured were systolic and diastolic blood pressure. The results of the sepat leaf extract study dose of 50 mg/KgBW can increase urine volume and can reduce blood pressure by the CODA® method. The conclusion from this research is that sepat leaf ethanol extract has antihypertensive activity.