ANTIINFLAMATION AND ACUTE TOXICITY OF ETLINGERA ELATIOR LEAVES EXTRACT

Abstract

Torch ginger leaves (Etlingera elatior) were proven to have health properties and widely used in community continuously. Phytochemical analysis of torch ginger leaves extract revealed the presence of flavonoid compounds and other polyphenol compounds that are known to have anti-inflammatory and inhibition of alfa glucosidase activity. Anti-inflamamatory activity was tested with the Human Red Blood Cell Stability (HRBC) method. Inhibition of red blood cell lysis due to induction of hypotonic solution was used to measure of anti-inflammatory activity. The toxicity activity of torch ginger leaves was to determine its safety, with LD50 value as a parameter. Torch ginger leaves were extracted using 70% ethanol and concentrated into a thick extract. Anti-inflammatory activity was expressed in IC50 value, which mean the ability of extract to inhibit 50% lysis of red blood cells as a marker of inflammation. IC50 value as antiinflammation activity of leaves extract was 71.24 μg/mL, while the IC50 value of diclofenac sodium as a standard was 48.94 μg /mL. Acute toxicity of leaves extract with oral acute toxicity method was used female mice at aged 8-12 weeks and were grouped randomly.After 14 days of observation, a biochemical examination was carried out and the animals were sacrificed for organ weighing and histopathological examination. The results showed that no deaths or strange behavior were observed during acute toxicity testing. Organ weight and clinical chemical parameters including SGOT, SGPT and creatinine showed no significant difference (p <0.05) when compared to the control group. Torch ginger leaves extract hadpotential antiinflamation properties and showed LD50> 2000mg / kgBW. It is still safe for human consumption.